NHSRC Product Updates

The US EPA National Homeland Security Research Center (NHSRC) has just published the most recent list ofproduct updates. 


1) Biological Sample Preparation Collaboration Project, Detection of Bacillus anthracis Spores in Soil

Report:  The objective of this work was to develop and optimize an automated immunomagnetic separation (AIMS) method for isolation of Bacillus anthracis (BA) spores from soil. In this study, the AIMS method for separating BAspores from various soil matrices was developed using the Dynabeads® technology (Invitrogen Corporation, Carlsbad, CA). This involved optimizing paramagnetic beads conjugation with antibodies specific to BA spores, designing an automated program (BeadRetriever™, Invitrogen) and developing standard procedures for separating BAspores from four soil types using AIMS.

2) Biological Sample Preparation Collaboration Project, Detection of Bacillus anthracis Spores in Soil

Journal Article:  A method was developed to deposit Bacillus subtilis spores via aerosolization onto various surface materials for biological agent decontamination and detection studies.

3) Technology Evaluation Report Real UV254 Security Monitor

Technology Evaluation Report:  EPA recently evaluated the performance of several online total organic carbon (TOC) analyzers and ultraviolet spectrometers including the Real UV254 Security Monitor. The primary objective of this evaluation was to determine the response of the TOC analyzers and ultraviolet spectrometers upon the introduction of contaminants such as toxic industrial chemicals (TICs) and biological contaminants in drinking water.


1) Evaluation of Liquid and Foam Decontamination Technologies for Surfaces Contaminated by Bacillus anthracis Spores

Summary:  Summary of the results of multiple studies on the effectiveness of liquid and foam decontamination technologies against Bacillus anthracis spores

2) Efficacy of liquid spray decontaminants for inactivation of Bacillus anthracis spores on building and outdoor materials

Journal Article:  The study results may be useful in the selection of technologies to decontaminate buildings and outdoor areas in the event of contamination with B. anthracis spores. These results may also facilitate selection of decontaminant liquids for the inactivation of other spore forming infectious disease agents.

3) Reports on the evaluation of technologies to remove radionuclides from concrete surfaces

+ Technology Evaluation Report CS Unitec ETR180 Circular Sander for Radiological Decontamination;

+ Technology Evaluation Report Empire Abrasive Blast N’Vac for Radiological Decontamination;

+ Technology Evaluation Report Industrial Contractors Supplies, Inc. Surface Dust Guard with Diamond Wheel for Radiological Decontamination;

+ Technology Evaluation Report Industrial Contractors Supplies, Inc. Surface Dust Guard with Wire Brush for Radiological Decontamination;

+ Technology Evaluation Report Real UV254 Security Monitor; and

+ Technology Evaluation Report River Technologies LLC 3-Way Decontamination System for Radiological Decontamination


1) CANARY USER’S MANUAL and software upgrades

Software:  For initial download please contact Terra Haxton   haxton.terra@epa.gov.  The goal of CANARY is to take standard water-quality data and use mathematical algorithms to identify the onset of periods of anomalous water quality, while at the same time, limiting the number of false alarms that occur. The working definition of “anomalous” can be set by the user by selecting the configuration parameters. These parameters may vary from one utility to the next and may even vary across monitoring locations within a single utility. CANARY can be set up to receive data from a SCADA database, and return alarms to the SCADA system.

2) EPANET Multi-Species Extension Software

Software:  EPANET is used in homeland security research to model contamination threats to water systems. Historically, EPANET has been limited to tracking the dynamics of a single chemical transported through a network of pipes and storage tanks, such as a fluoride used in a tracer study or free chlorine used in a disinfection decay study. Recently, the NHSRC released a new extension to EPANET called EPANET-MSX (Multi-Species eXtension) that allows for the consideration of multiple interacting species in the bulk flow and on the pipe walls. This capability has been incorporated into both a stand-alone executable program as well as a toolkit library of functions that programmers can use to build customized applications.

3) Threat Ensemble Vulnerability Assessment- Sensor Placement Optimization Tool (TEVA-SPOT) Graphical User Interface (EPA/600/R-08/147)

Software:  The ultimate aim of a simulation and its subsequent analysis is to determine the vulnerability of the distribution system to contaminant releases and determine the optimal locations to place a set of water quality sensors in the network to mitigate the impacts of contamination. This tutorial helps to familiarize users to the TEVA-SPOT functionality.

4) Development of a Sensitive Detection Method for Stressed E. coli O157:H7 in Source and Finished Drinking Water by Culture-qPCR

Journal article:  A sensitive and specific method that also demonstrates viability is of interest for detection of E. coli O157:H7 in drinking water. A combination of culture and qPCR was investigated. Two triplex qPCRs, one from a commercial source and another designed for this study were optimized from 5 different assays to be run on a single qPCR plate.


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